| 黑曲霉SS-A-52液体发酵生产果胶酶培养基优化的研究 |
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| 引用本文： | 孙维国,李统锋. 黑曲霉SS-A-52液体发酵生产果胶酶培养基优化的研究[J]. 石油化工应用, 2004, 23(4): 7-10 |
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| 作者姓名： | 孙维国 李统锋 |
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| 作者单位： | 宁夏夏盛实业集团有限公司,银川,750002 |
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| 摘 要： | 采用一株产果胶酶的黑曲霉菌种SS-A-52进行了液体发酵生产果胶酶的培养基优化,研究结果表明:在初始pH5.4、培养温度28℃-30℃的条件下,最佳培养基组成为:3%的桔皮粉,2%的麸皮,0.35%硫铵,0.2%的碳酸钙及少量的营养液。其产果胶酶活力可这10.46万μ/ml,通过尝试过程分批补料工艺,产酶达18.59万μ/ml。
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| 关 键 词： | 果胶酶 液体深层发酵 黑曲霉 |
| 文章编号： | 1672-3058(2004)04-04 |
| 修稿时间： | 2004-10-15 |
Studies on the Optimal Culture of Submorged Liquid Fermentation Producing Pectinase by Aspergillus niger SS- A- 52 |
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| SUN Weiguo,LI Tongfeng. Studies on the Optimal Culture of Submorged Liquid Fermentation Producing Pectinase by Aspergillus niger SS- A- 52[J]. Petrochemical Industry Application, 2004, 23(4): 7-10 |
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| Authors: | SUN Weiguo LI Tongfeng |
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| Affiliation: | Ningxia SunSon Industry Group.Ltd.Co.750002 |
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| Abstract: | The optimal culture of submerged liquid by using a strain of ligh producing pectinase AspergiUus niger SS- A-52.The resells indicated the optimal cultural compound was 3% orange bran 0.35%(NH_4)_2SO_4 0.2% CaCO_3 2% wheat bran and a bit of mineral material.Under the conditions,Asper.nigerSS-A-52 could accumulate pectinase 1.064×10~5μ/ml.According to feed batch culture in fermentation process,pectinase acctivity reached 1.859×10~5u/ ml. |
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| Keywords: | pectinase submerged liquid fermentation Aspergillus niger SS-A-52 |
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