响应面法优化无花果中蛋白的提取条件

采用单因素试验方法,研究磷酸盐缓冲液浓度、料液pH、固液比对无花果中蛋白提取量的影响,并利用Design-Expert软件响应面分析法构造模型,确定无花果中蛋白的最优提取条件为:磷酸盐缓冲液浓度0.08 mol/L,pH 7,固液比1∶50,无花果蛋白提取量的最优预测值为9.633 27 mg/g,试验验证,所得蛋白提取量的实际值为9.475 0 mg/g。

Research on Optimal Extraction Condition of Protein from Ficus Carica

To optimize the extraction condition of protein from ficus carica,the effects of phosphate concentration,pH,ratio of solid and liquid phase were investigated by single-factor experiment.Response surface methodology from Design-Expert software was employed to formulate a model to optimize extraction condition.The results showed that the phosphate concentration was 0.08 mol/L,the pH was 7,the ratio of solid and the liquid phase was 1∶50,the optimization value could be obtained and the predicted value was 9.633 27 mg/g,under the same condition,the actual extraction quantity of protein was 9.475 0 mg/g.