Quantitative analysis of polyenoic phospholipid molecular species by high performance liquid chromatography |
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Authors: | Hitoshi Takamura Hiroshi Narita Reiko Urade Makoto Kito |
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Affiliation: | (1) Research Institute for Food Science, Kyoto University, 611 Uji, Kyoto, Japan |
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Abstract: | The quantitative analysis of phospholipid molecular species containing polyenoic fatty acids is described. Dinitrobenzoyl
derivatives of diacylglycerols prepared from phospholipids were separated into individual molecular species by reversed-phase
high performance liquid chromatography (HPLC) using a combination of two solvent systems and were quantified at 254 nm. Thirty-six
molecular species were resolved from the phosphatidylcholines of rat hearts, human platelets and Chinese hamster V79-R cells.
The derivatives of alkenylacyl molecular species from platelet phosphatidylethanolamine were resolved concomitantly with diacyl
molecular species. |
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