| 保健品中牛羊源性成分的PCR检测 |
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| 引用本文： | 陈颖,钱增敏,徐宝梁,吴亚君,王晶,苏宁. 保健品中牛羊源性成分的PCR检测[J]. 食品科学, 2004, 25(10): 215-218 |
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| 作者姓名： | 陈颖 钱增敏 徐宝梁 吴亚君 王晶 苏宁 |
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| 作者单位： | 中国检验检疫科学研究院,北京,100025 |
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| 基金项目： | 人事部留学人员科技活动重点资助项目 |
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| 摘 要： | 本文根据牛、羊线粒体mtDNA中的保守序列，设计了针对牛羊源性成分检测的特异性扩增引物，通过聚合酶链反应技术(Polymerase Chain Reaction，PCR)建立了保健品中牛羊源性成分的快速检测方法。通过内切酶DpnⅡ和SspⅠ可分别对牛羊成分的扩增结果进行进一步验证，该方法对牛源性成分的检测低限为0．05％，对山羊和绵羊源性成分的检测低限分别为0．005％和和0．5％，可作为保健品中牛羊源性成分检测有效方法，也可作为保健品及动物源性产品中成分真伪鉴别的准确、可靠方法。
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| 关 键 词： | 保健品 牛源性成分 羊源性成分 PCR检测 |
| 文章编号： | 1002-6630(2004)10-0215-04 |
Species-specific PCR for Identification of Horse and Donkey Materials in Feedstuff |
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| CHEN Ying,QIAN Zeng-ming,XU Bao-liang,WU Ya-jun,WANG Jing,SU Ning. Species-specific PCR for Identification of Horse and Donkey Materials in Feedstuff[J]. Food Science, 2004, 25(10): 215-218 |
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| Authors: | CHEN Ying QIAN Zeng-ming XU Bao-liang WU Ya-jun WANG Jing SU Ning |
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| Abstract: | This study described a rapid PCR assay for the detection of bovine and sheep materials in functional food. Species-specific PCR primer had been used for this purpose and the enzymes DpnII and SspI were used to certify the PCR result for bovineand sheep respectively, the detection limitation was 0.05% for bovine derived material and 0.005% and 0.5% for goat and sheeprespectively. It could be one of an effective method for detection bovine and sheep derived materials and for verifying theauthenticity of the component of functional food. |
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| Keywords: | functional food bovine derived material sheep derived material PCR assay |
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