高胶质食品中合成色素检测方法的改进

采用用聚酰胺吸附法、碱液直接提取法、碱性醇液提取法、饱和硼砂沸腾水浴提取法、海砂研磨法5种提取方法分别提取凤爪中的柠檬黄、苋菜红、胭脂红、日落黄、诱惑红和亮蓝6种色素,并用超高效液相色谱法测定。结果表明:5种提取方法中,海砂研磨法对凤爪中6种色素的提取效率最高;用选定的方法对超市中抽检的25个凤爪样品进行检测,发现样品中主要含有柠檬黄、日落黄和诱惑红,含少量胭脂红,不含苋菜红和亮蓝。

Improvement on the Determination of Synthetic Pigment from High Gelatine Foodstuff by UPLC

In this experiment,synthetic pigments were extracted from high gelatine foodstuff(chicken claws) by 5 different extraction methods: polyamide adsorption,alkaline solution,alkaline ethanol,saturated borax solution and boiling water extraction,grinding silicon dioxide.The pigments were measured by UPLC.The results showed:grinding silicon dioxide is the most efficient method to extract six synthetic pigments in chicken claws;Twenty-five chicken claws from supermarkets were tested with this method,only tartrazine,sunset yellow,allura red and a bit of ponceau 4R were found,other two synthetic pigments were not detected.Therefore,grinding silicon dioxide method is good on ensuring extracting variety and quantity of synthetic pigments.This method also greatly simplified experimental processes.