| 白腐菌Pleurotus eryngii-Co007产木质素降解酶条件的优化 |
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| 引用本文： | 陈敏,郭倩,姚善泾.白腐菌Pleurotus eryngii-Co007产木质素降解酶条件的优化[J].食品与发酵工业,2011,37(1). |
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| 作者姓名： | 陈敏 郭倩 姚善泾 |
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| 作者单位： | 1. 浙江工商大学食品与生物工程学院,浙江,杭州,310012
2. 浙江大学化学工程与生物工程学系,浙江,杭州,310027 |
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| 摘 要： | 木质素降解酶是参与降解木质素的酶类,广泛存在于白腐真菌中。为提高白腐菌Pleurotus eryngii-Co007的产木质素降解酶的活力,研究了该菌株的产酶曲线、发酵参数及培养基组分对其产木质素降解酶的影响。单因素试验结果表明该菌株发酵第8天时发酵液中木质素降解酶酶活最高;较优的发酵参数是pH 5.0~6.0、发酵温度25~27℃,接种量10%~15%,其中pH影响最显著;麦芽糖和有机氮源有利于木质素降解酶的表达。但考虑到酶表达的增加量和生产成本的经济性选择3%的葡萄糖、0.2%酵母粉和0.2%牛肉膏组合氮源;诱导剂中秸杆、Cu2+和吐温80诱导产酶效果明显。
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| 关 键 词： | Pleurotus eryngii-Co007 木质素降解酶 产酶条件 优化 |
Optimization of the Conditions for Ligninolytic Enzymes Production from White Rot Fungi Pleurotus eryngii-Co007 |
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| Chen Min,Guo Qian,Yao Shan-jing.Optimization of the Conditions for Ligninolytic Enzymes Production from White Rot Fungi Pleurotus eryngii-Co007[J].Food and Fermentation Industries,2011,37(1). |
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| Authors: | Chen Min Guo Qian Yao Shan-jing |
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| Affiliation: | Chen Min1,Guo Qian1,Yao Shan-jing21(College of Food Science & Bioengineering,Zhejiang Gongshang University,Hangzhou 310012,China)2(Department of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China) |
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| Abstract: | Ligninolytic enzymes involved in lignin biodegradation are mainly produced by white-rot fungus.In order to enhance the ligninolytic enzymes activity of Pleurotus eryngii-Co007,the ligninolytic enzymes production curve and the influence on igninolytic enzymes production by fermentation conditions were studied.Pleurotus eryngii-Co007 was found to produce maximum amount of ligninolytic enzymes at the eighth day.The results of single factor tests were listed as follows: the optimum fermentation parameters were ... |
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| Keywords: | Pleurotus eryngii-Co007 |
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