| 动态高压微射流对聚半乳糖醛酸酶的影响 |
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| 引用本文： | 黄波,钟业俊,刘成梅,刘伟,尹曼.动态高压微射流对聚半乳糖醛酸酶的影响[J].食品与机械,2011,27(2). |
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| 作者姓名： | 黄波 钟业俊 刘成梅 刘伟 尹曼 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室,江西,南昌,330047;南昌大学生物质转化教育部工程研究中心,江西,南昌,330047 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 利用动态高压微射流(DHPM)处理聚半乳糖醛酸酶(PG),通过对酶活、圆二色谱、紫外光谱和巯基含量的测定,研究DHPM对PG酶活性和构象的影响.结果显示,经75,125,175 MPa的动态高压微射流处理后,PG酶的相对活性分别为93.2%、95.3%和91.2%;125 MPa分别处理2、3次后,PG酶的相对活性分别为97.4%、88.2%.分子构象分析表明,经不同压力DHPM处理后,PG酶的β-折叠含量有不同程度降低,PG 酶分子结构更为"松散",原来包埋在分子内部的TrP和Tyr残基部分暴露出来,分子表面的二硫键被打断形成巯基,但从分子整体来看,新的二硫键形成的量要多于被打断的量.
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| 关 键 词： | 动态高压微射流技术 聚半乳糖醛酸酶 酶活力 构象 |
Effect of dynamic high-pressure microfluidization on polygalacturonase |
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| HUANG Bo,ZHONG Ye-jun,LIU Cheng-mei,LIU Wei,YIN Man.Effect of dynamic high-pressure microfluidization on polygalacturonase[J].Food and Machinery,2011,27(2). |
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| Authors: | HUANG Bo ZHONG Ye-jun LIU Cheng-mei LIU Wei YIN Man |
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| Affiliation: | HUANG Bo1,2 ZHONG Ye-jun1,2 LIU Cheng-mei1,2 LIU Wei1,2 YIN Man1,2(1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang,Jiangxi 330047,China,2.Engineering Research Center of Biomass Conversion,Ministryof Education,China) |
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| Abstract: | The effects were observed of dynamic high pressure microfluidization treatment on the activity and conformation of polygalacturonase(PG).Activity of PG,far-UV circular dichroism,UV absorption and sulphydryl groups detection were employed.The results showed: Under the pressures of 75 MPa,125 MPa and 175 MPa,PG activity were remained 93.2%,95.3% and 91.2%,respectively;after treated under 125 MPa for 2 and 3 passes,97.4% and 88.2% of PG activity were remained,respectively.The analysis of conformation indicated... |
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| Keywords: | dynamic high-pressure microfluidization polygalacturonase activity conformation |
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