锌离子对菖蒲的胁迫反应研究

采用水培法,以Hoagland溶液为基础培养液,研究Zn2+胁迫对菖蒲幼苗生长状况、细胞渗透性、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、叶绿素以及脯氨酸质量比的影响。结果表明,Zn2+胁迫下菖蒲幼苗生长受到严重影响;随着Zn2+质量比的增加,叶片电导率不断增大;POD、SOD活性均高于对照,并且呈现先升高后降低的正相关趋势;叶绿素的质量比显著降低,与Zn2+质量比呈负相关趋势;脯氨酸质量比则一直呈上升的变化趋势。研究结果说明,酶活性的升高是由于菖蒲对Zn2+胁迫做出的应激反应,而高质量比Zn2+却对菖蒲的保护酶活性有抑制作用。

Response of Acorus calamus to Zn2+ stress

Acorus calamus was cultured in Hoagland＇ s solution. The effects of Zn^2 ＋ stress on the growth of Acorus calamus, the cell permeability, the activity of peroxidase （POD） and superoxide dismutase （SOD） and the chlorophyll and proline concentrations were investigated. The results show that the growth of Acorus calamus was badly influenced and the leaves＇ conductivity increased. The activity of POD and SOD first increased, then decreased, but was higher than that in the control experiment. The chlorophyU concentration dramatically decreased and was negatively related with the Zn^2 ＋ concentration. The proline concentration increased along with the Zn^2 ＋ concentration. The results indicate that the increase of enzyme activity is an adaptation response of Acorus calamus stressed by Zn^2 ＋, while a high concentration of Zn^2 ＋ inhibits the enzyme activity.