| 酶法反应液中谷氨酰胺的提取工艺研究 |
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| 引用本文： | 刘金山,段作营,毛忠贵. 酶法反应液中谷氨酰胺的提取工艺研究[J]. 食品与发酵工业, 2004, 30(12): 77-79 |
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| 作者姓名： | 刘金山 段作营 毛忠贵 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,无锡,214036 |
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| 摘 要： | 根据谷氨酸和谷氨酰胺的电离常数的差异,针对谷氨酰胺酶法反应液的性质,采用单根强碱性阴离子Cl-型拄,从酶法反应液中提取谷氨酰胺。经预处理、脱色、离交分离、浓缩结晶,反应液中谷氨酰胺总提取收率为519%,纯度为968%,谷氨酸去除率964%。
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| 关 键 词： | 谷氨酰胺 离子交换 分离 提取 |
| 修稿时间： | 2004-06-30 |
Studies on the Extraction of Glutamine from Enzyme Broth Produced Glutamine |
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| LIU Jinshan,Duan Zuoying,Mao Zhonggui. Studies on the Extraction of Glutamine from Enzyme Broth Produced Glutamine[J]. Food and Fermentation Industries, 2004, 30(12): 77-79 |
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| Authors: | LIU Jinshan Duan Zuoying Mao Zhonggui |
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| Abstract: | The separation of glutamine produced by enzyme method was developed by using one-step anion exchange based on the difference among the ionization constants of glutamic acid (Glu), glutamine(Gln) and the characteristics of enzyme broth produced glutamine. When the broth was treated with the flocculation, decoloration, ion exchange, concentration and crystallization, the recovery rate of Gln was 51.9%, the purity reached 96.8%, and the removal rate of Glu was up to 96.4%. |
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| Keywords: | glutamine ion exchange separation extraction |
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