小鼠脾细胞中低剂量辐射诱导产物及其生物学活性

采用不同的低剂量辐射（LDR）剂量、LDR诱导产物用量、照射方式、照射后蛋白提取时间为处理因素，观测诱导产物生物学活性的变化。结果表明，用此提取液刺激后，小鼠脾脏细胞转化明显增高。经不同处理因素作用后同位素掺入游离小鼠脾细胞的每分钟衰变数（DPM）值有明显改变。实验证实，经5－15cGy LDR后2-16h都可从小鼠脾脏细胞中提取得到LDR诱导产物，诱导产物具有生物学活性，可刺激脾脏细胞转化，但是，其活性受到多种因素的影响。进一步的分析表明，此诱导产物提取液的有效成分很可能为LDR诱导的新生蛋白质。

LOW DOSE RADIATION INDUCED PRODUCTS IN MOUSE SPLEEN AND THEIR BIOLOGICAL ACTIVITY

Low dose radiation (LDR) induced products were extracted from mouse spleen and its biological activity was preliminarily detected. The mouse spleen cells were sonicated and the induced products in the lysate were obtained with centrifugation. The method of radioactive isotope incorporation was used and the DPM as an index for evaluating the biological activity of the induced products, To investigate the possible influences of variant factors on the activity of the products, tests were carried out under different conditions: different time for extraction, different radiation doses, different volumes of extracted products for stimulation, and different radiation modes. Results showed that the transformation ability of mouse spleen cells was significantly enhanced after stimulation of these cells with LDR-induced products. And the above-mentioned changes in experimental conditions could influence the activity of the induced products to different extents. It was indicated from this study that the active LDR-induced products could be extracted 2-16h after irradiation with a dose range from 5 to 15cGy, and the effective composition of the induced products maybe belonged to newly synthesized protein.