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| 抗黄曲霉毒素M1免疫亲和柱的制备 | |
| 引用本文： | 孙兴荣,裴世春,柳家鹏,王莹. 抗黄曲霉毒素M1免疫亲和柱的制备[J]. 现代食品科技, 2011, 27(3): 306-309 |
| 作者姓名： | 孙兴荣 裴世春 柳家鹏 王莹 |
| 作者单位： | 1. 黑龙江八一农垦大学食品学院,黑龙江大庆,163319 2. 齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔,161006 3. 北京华安麦科生物技术有限公司,北京,102200 |
| 基金项目： | 黑龙江省留学归国人员基金 |
| 摘 要： | 制备黄曲霉毒素M1污染样品的净化前处理免疫亲和柱.利用无血清培养基制备的高纯度抗黄曲霉毒素M1单克隆抗体和溴化氰活化的Sepharose 4B进行偶联、装柱后通过IC-ELSIA对免疫亲和柱的性能评价确立了最佳反应条件.免疫亲和柱的有效柱容量为500ng,经添加回收试验测定的平均回收率为92.46%0,变异系数2.3%... |
| 关 键 词： | 黄曲霉毒素M1 免疫亲和柱 无血清培养基 |
Preparation of Immunoaffinity Column for Anti-AFM1 |
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| SUN Xing-Rong,PEI Shi-Chun,LIU Jia-Peng,WANG Ying. Preparation of Immunoaffinity Column for Anti-AFM1[J]. Modern Food Science & Technology, 2011, 27(3): 306-309 | |
| Authors: | SUN Xing-Rong PEI Shi-Chun LIU Jia-Peng WANG Ying |
| Affiliation: | 1.Food Science College,Heilongjiang August First Land Reclamation University,Daqing 163319,China)(2.Food and Biological Engineering College,Qiqihar University,Qiqihar 161006,China)(3.Beijing Hua’an Magnech Bio-Tech Co.,Ltd,Beijing 102200,China) |
| Abstract: | Preparation of aflatoxin M1 immunoaffinity column for clean-up contaminated samples.The high-purity anti-aflatoxin M1 monoclonal antibody with serum-free medium was prepared and coupled with CNBr activated Sepharose 4B,and then packed in a column.Then the column was evaluated through IC-ELISA and established the optimum reaction conditions.The capacity of immunoaffinity column was 500 ng,the average recovery of aflatoxin M1 was 92.46%;and the variation coefficients were 2.3%~7.2%.The immunoaffinity columns was prepared by coupling high-purity anti-aflatoxin M1 monoclonal antibody with CNBr Activated Sepharose 4B can be applied to detect contamination samples fast,such as aflatoxin M1 in dairy products. |
| Keywords: | aflatoxin M1 immunoaffinity column serum-free medium |
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