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压热-酶法制备碎米抗性淀粉的工艺及其结构特性研究
引用本文:吴红引,王泽南,张秋子,李莹,刘鹏.压热-酶法制备碎米抗性淀粉的工艺及其结构特性研究[J].现代食品科技,2011,27(2):166-169,165.
作者姓名:吴红引  王泽南  张秋子  李莹  刘鹏
作者单位:合肥工业大学生物与食品工程学院,安徽合肥,230009
基金项目:安徽省教育厅自然科学重点项目
摘    要:研究以碎米为原料压热-酶法制备抗性淀粉的工艺.通过单因素和正交试验,获取最佳工艺条件:淀粉浆质量浓度20%、压热温度125℃、压热时间40 min、普鲁兰酶添加量3.5 U/g干淀粉,酶解时间6 h.在此条件下碎米抗性淀粉得率为16.8%;采用扫描电镜观察分析表明碎米抗性淀粉具有更稳定的晶体结构,具有抗酶解性;运用X-...

关 键 词:碎米  抗性淀粉  压热  普鲁兰酶  工艺  结构特性

Enzymatic Pressure-heating Preparation of Broken Rice Resistant Starch and its Structural Properties
Wu Hong-yin,WANG Ze-nan,ZHANG Qiu-zi,LI Ying,LIU Peng.Enzymatic Pressure-heating Preparation of Broken Rice Resistant Starch and its Structural Properties[J].Modern Food Science & Technology,2011,27(2):166-169,165.
Authors:Wu Hong-yin  WANG Ze-nan  ZHANG Qiu-zi  LI Ying  LIU Peng
Affiliation:(School of Biotechnongy and Food Engineering,Hefei University of Technology,Hefei 23009,China)
Abstract:Enzymatic pressure-heating preparation of broken rice resistant starch was studied and optimized through single factors and orthogonal tests as follows:20 g/mL of starch slurry concentration,125℃of autoclaving temperature,40 min of holding time,3.5 U pullulanase per gram of dry starch and 6 h of enzymolysis.Yield of resistance starch was 16.8%under these conditions.Scanning electron microscope observation indicated that the broken rice resistant starch had the stable crystal structure with anti-enzymolysis property.X-ray diffraction of its crystallinity showed that broken rice starch was of A type,while the broken rice resistant starch’s crystallization structure was of the coalition of B and V types.Those indicated that the resistant starch was the stodginess.
Keywords:broken rice  resistant starch  terilizing boiler  pullulanase  technongy  structural properties
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