重组人胰高血糖素类多肽-1(7-36)对正常动物降糖作用的实验研究

目的 观察rhGLP-1(7-36) 对正常动物的降血糖和促胰岛素分泌作用以及该作用的葡萄糖依赖性。 方法 用不同动物模型sc 不同剂量的rhGLP-1(7-36) 后, 收集血清, 测定血糖值及胰岛素值。 结果 正常C57 小鼠和家兔sc 不同剂量的rhGLP-1(7-36) 后, 血糖和胰岛素分泌均无明显改变, 提示rh-GLP-1(7-36) 并不影响血糖正常动物的胰岛素分泌及血糖;对于给糖负荷动物, rhGLP-1(7-36) 使其胰岛素分泌增加, 并进而使升高的血糖明显降低, 表明rhGLP-1(7-36) 具有明显的葡萄糖依赖的促胰岛素分泌作用。在C57 小鼠和家兔糖耐量实验中发现, rh-GLP-1(7-36) 16 μg·kg-1使糖负荷家兔胰岛素分泌增加, 血糖升高曲线明显降低, 说明rhGLP-1(7-36) 能明显增加动物对糖的耐受。 结论 rhGLP-1(7-36) 通过葡萄糖依赖的胰岛素分泌作用降低过高血糖, 而不降低正常血糖, 同时用量小, 起效快, 安全性高。

Effects of glucagon-like peptide-1 (7-36) on decreasing blood glucose in normal animals

AIM: To observe the effects of glucagonlike peptide-1 (rhGLP-1) (7-36) on decreasing blood glucose and stimulating the insulin secretion in mice and rabbits, and how the actions depend on the glucose levels. METHODS: After subcutaneous injection of rhGLP-1(7-36) at different doses in different animal models, the serum was collected and the values of blood glucose and insulin were measured. RESULTS: After subcutaneous injection of rhGLP-1 (7-36) at different doses in normal C57 mice and rabbits, no obvious changes of the levels of blood glucose and insulin secretion was found, which suggested rhGLP-1 (7-36) did not affect the levels of blood glucose and the action of insulin secretion.For the mice and rabbits given doses of glucose 2 and 3 g·kg^-1 respectively, rhGLP-1 (7-36) had the effects of increasing insulin secretion, and lowering the level of blood glucose strongly, which suggested rhGLP-1(7-36) had obvious effect of increasing insulin secretion depending on the level of glucose.In the glucose tolerance test (GTT), rhGLP-1 (7-36) of 16 μg·kg^-1 increased the insulin secretion of rabbit given glucose, and had the effect of lowering the glucose curve obviously, which showed the rhGLP-1(7-36) could improve the tolerance to glucose of rabbits. CONCLUSION: rhGLP-1 (7-36) has the effect of lowering the levels of glucose.However, it has no effect on the normal level of glucose.