桂枝汤有效部位A对IL-1剌激的大鼠脑微血管内皮细胞前列腺素E2及其主要信号转导元件的影响

目的: 探讨桂枝汤解热有效部位A(Fr.A)对IL-1刺激的大鼠脑微血管内皮细胞(rCMEC)PGE₂信号转导通路主要元件的影响。方法: 制备大鼠含药血清,通过放射免疫法测定PGE₂含量,酶反应底物法测定PLA₂活性,ELISA法测定C0X活性,比色法测定NO含量。结果: Fr. A含药血清处理iCMEC后,在IL-1刺激下,孵育液中PQE₂和NO含量、总C0X和C0X-2活性均显著减低,对升高的SPLA₂活性无明显影响。结论: Fi.A可通过影响PGE₂信号转导通路主要元件C0X及N0,进而影响PGE₂水平。

Effects of Fr. A in Guizhi Tang on the excretion of PGE2 and its signal transduction components in cerebral microvascular endothelial cells stimulated by IL-1

AIM: To investigate the effects of Fr. A, an antipyretic active fraction extracted from Guizhi Tang, on the excretion of PGE₂ and its signal transduction com-ponents in cerebral microvascular endothelial cells (rCMEC) stimulated by IL-1. METHODS: Fr. A-containing rat serums were prepared. The PGE₂, PLA₂, COX and NO were measured by radioimmunoassay, enzyme reaction substrate assay, ELISA and colorimetry, respectively. RESULTS: When administration of Fr. A-containing serum, contents of PGEj and NO, the activity total COX and of COX-2 were significantly decreased in rCMEC. But the activity of sPLA₂ was not significantly decreased. CONCLUSION: Fr. A can affect the level of PGE₂ by iniluencing the main elements of COX and NO in PGE₂ signal transduction passway.