当归注射液对烟酒所致大鼠宫内发育迟缓的拮抗作用及机制

目的:了解烟酒所致胎儿宫内发育迟缓(IUGR)时孕鼠胎盘组织抗氧化功能的改变, 探讨当归注射液的保护作用及机制。方法:采用烟酒混合因素建立大鼠IUGR 模型, 部分孕鼠给予当归注射液0.25 g·kg^-1治疗, 测定胎盘组织中还原型谷胱甘肽(GSH) 和丙二醛(MDA) 的含量及超氧化物歧化酶(SOD)、过氧化氢酶(Cat) 和谷胱甘肽过氧化物酶(GSH-Px) 的活性。结果:IUGR 孕鼠胎盘组织MDA和GSH 含量均明显增加(P <0.01, P <0.05), SOD、GSH-Px 和Cat 活性显著下降(P <0.01, P <0.05) 。经当归注射液治疗后, 胎儿宫内生长发育接近正常,上述指标也得到改善。结论:胎盘氧化损伤可能是IUGR发病的一个重要机制。当归注射液能提高IUGR孕鼠胎盘的抗氧化能力, 促进胎儿宫内生长发育。

Antagonizing effects and mechanism of Chinese angelica liquid injection on intrauterine growth retardation-induced by smoking and drinking alcohol in rats

AIM: To evaluate the changes of antioxidative function of placenta with intrauterine growth retardation (IUGR) in rats and to find out the effect and mechanism of Chinese angelica liquid injection.METHODS: The model of IUGR rat was made by means of passive smoking and drinking alcohol.Some pregnant rats were treated with Chinese angelica liquid injection, the contents of malondialdehyde (MDA) and glutathione (GSH), and the activities of superoxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GSH-Px) in rat placenta were measured.RESULTS: The contents of MDA and GSH in IUGR group were higher than those in the normal control group (P <0.01 or P <0.05).Meanwhile, the activities of SOD, Cat and GSH-Px in IUGR group were lower than those in the normal control group (P <0.01 or P <0.05).These indexes were reversed after the treatment of Chinese angelica liquid injection, and fetal growth and development in treated group were near to those in the normal control group.CONCLUSION: Oxidative damage occurred in placenta may be an important mechanism of IUGR formation.Chinese angelica liquid injection can enhance antioxidative function of placenta and promote fetal growth and development.