7-硝基吲唑对兔异丙酚麻醉深度调节的研究

目的: 研冗7-硝基丨吐（7-nitro indazolei,7-NI)对兔异丙酚麻醉深度的调节作用。方法: 20只日本大耳兔,雌雄不限,随机分为2组(n=10):对照组经腹腔注射2 ml花生油;7-硝基吲唑组经腹腔注射50 mg·kg^-17-硝基吲唑。20 min后经颈外静脉连接Graseby 3 500注射泵进行靶控输注（TCI),以咀嚼反射消失作为浅麻醉标志,测定达浅麻醉状态所需异丙酚TCI血药浓度、双频指数（BIS)值及心率变异指数（HRVI)值。对照组达浅麻醉状态后再次经腹腔注射50 mg·kg^-17-硝基吲唑,观察BIS值、HR-VI值变化。结果: 50mg·kg^-17-硝基吲唑显著降低兔达浅麻醉状态时所需TCI农度。对照组达浅麻醉状态后再次注射50 mg·kg^-17-硝基吲唑麻醉深度表现加深趋势,但无显著的统计学意义（P>0.05)。结论: 7-硝基吲唑通过选择性抑制中枢nNOS活性增强异丙酚麻醉效能,提示中枢NO/cGMP信号转导途径在异丙酚全麻作用机制中发挥作用。异丙酚可能通过抑制中枢nNOS活性影响NO/cGMP信号转导系统。

Effects of 7-nitro indazole on anesthetic depths in rabbits induced by propofol

AIM: To investigate the regulative effects of 7-NI on anesthetic depths in rabbits induced by propo-fol. METHODS: Twenty Japanese large auris rabbits of both sexes were randomly divided into 2 groups (n=10 in each): control group was given 2 milliliter peanut oil, ip and trial group was given 50 mg·kg^-17-NI, ip. After twenty minutes of administration, propofol were target-controlled infused by Graseby 3 500 injection pump that connected with jugular vein. The loss of chewing was in-dicated the light anesthesia. The blood concentration of propofol and the bispectml index (BIS), and heart rate variability index(HRVI) were determined as the light an-esthetic depth reached. In order to observe the changes of BIS and HRVI, the control group was given 50 mg·kg^-17-NI, ip in the light anesthetic depth. RESULTS: 50 mg·kg^-17-NI significantly decreased the target blood concentrations in the light anesthetic depth. Administra-tion of 50 mg·kg^-1 7-NI increased the anesthetic depth after the light anesthetic depth reached (P>0.05). CONCLUSION: 7-NI can increase the anesthetic action of propofol by selectively inhibition of the activity of cen-tral nNOS, indicated that central NO/cGMP signal trans-duction pathway play an important role in the general an-esthesia machanism of propofol. Propofol may influence NO/cGMP signal transduction pathway by selectively inhi-bition of the activity of central nNOS.