牛磺酸对烫伤大鼠血浆及心肌组织肿瘤坏死因子和血管紧张素Ⅱ含量的影响

目的 观察牛磺酸对大鼠严重烧伤后心肌损害的影响。 方法 用健康Wistar 大鼠, 随机分为对照组、烧伤组(造成30 %TBSA Ⅲ度烫伤) 和治疗组(烧伤后腹腔注射牛磺酸, 剂量400 mg·kg^-1 体重)。于伤后1、3、6、12 和24 h 检测血浆TNF-α、Ang Ⅱ和TnT含量与心肌中TNF-α、Ang Ⅱ含量。 结果 烧伤大鼠烧伤后3 h血浆cTnT 呈显著升高, 12 h 达峰值, 24 h显著高于对照组;烫伤后6 h 血浆和心肌组织TNF-α含量显著高于对照组(P <0.01), 12 h 达峰值;伤后1 h 血浆Ang Ⅱ含量呈显著升高, 伤后24 h 血浆及心肌中Ang Ⅱ高于对照组;牛磺酸治疗组血浆cTnT、TNF-α和Ang Ⅱ水平较烧伤组均有显著性降低。血浆TNF-α、Ang Ⅱ含量与血浆cTnT 变化密切相关。 结论 牛磺酸对严重烫伤后心肌损伤有较好的保护作用。

Effect of taurine on the content of tumor necrosis factor alpha and angiotensin Ⅱ in plasma and myocardium of the rats following severe burns

AIM: To investigate the effect of taurine on myocardial injury in rats with severe burns. METHDOS: 110 healthy adult Wistar rats were randomly divided into 3 groups:the normal control (n=10, without burns), the burn group (n=50, subjected to a 30 % total body surface area III degree scald injury), and the group treated with taurine (n=50), by abdominal cavity injection taurine (400 mg·kg^-1) after scald injury immediately.Levels of tumor necrosis factor alpha (TNF-α), troponin (cTnT) and angiotensin Ⅱ (Ang Ⅱ) in plasma and content of TNF-α, Ang Ⅱ in myocardiumwere assayed at 1, 3, 6, 12 and 24 h postburn. RESULTS: Plasma cTnT increased markedly at 3h postburn, peaking at 12 h, and it was still higher than that of the control at 24 h postburn.TNF-αin plasma and myocardium also increased markedly at 6 h postburn, peaking at 12 h postbrun, and decreased somewhat at 24 h postburn, but it was still higher than that of the control group.Plasma Ang Ⅱ increased significantly at 1 h postburn, peaking at 6 h postburn, and it was also higher than that of the control in plasma and myocardium at 24 h postburn.After treated with taurine, however, levels of cTnT, TNF-αand Ang Ⅱ in plasma and myocardium decreased significantly compared with that of the burn group.Additionally, the levels of TNF-αand Ang Ⅱ in plasma were correlated with cTnT levels. CONCLUSION: Taurine can protect myocardium from injury following severe burns.