灵芝多糖肽对小鼠巨噬细胞自由基的清除作用

目的: 研究灵芝多糖肽对小鼠腹腔巨噬细胞自由基的清除作用。方法: 用四氧嘧啶、叔丁基氢过氧化物(tBOOH) 为氧化剂, 分别在小鼠体内、体外损伤小鼠腹腔巨噬细胞, 以DCHF-DA 为荧光指示剂,用共聚焦显微镜观察巨噬细胞的荧光变化, 并用共聚焦显微镜作时间系列扫描, 观察巨噬细胞荧光的动态变化。结果: 四氧嘧啶(75 mg·kg^-1, iv) 、叔丁基氢过氧化物(7.76 ×10^-5 mol·L^-1) 可造成巨噬细胞的氧化损伤, 使荧光密度增加, 灵芝多糖肽可减轻其损伤, 使荧光密度减少。时间系列扫描显示:随时间改变, 灵芝多糖肽可减少静息状态下小鼠腹腔巨噬细胞荧光密度, 也可减少由PMA(50 nmol·L^-1) 诱导的呼吸爆发状态下小鼠腹腔巨噬细胞荧光密度。结论: 灵芝多糖肽具有抗氧化作用, 对小鼠腹腔巨噬细胞自由基有清除作用。

Free radical scavenging activity by Ganoderma lucidum polysaccharides peptide on peritoneal macrophages in mice

AIM: To investigate the free radical scavenging activity of Ganoderma lucidum polysaccharides peptide (GLPP) on peritoneal macrophages in mice.METHODS: Alloxan and tert-butylhydroperoxide (tBOOH) were used as an oxidant to injury peritoneal macrophages in vivo in mice or in vitro, respectively. 2', 7'-dichlorodihydrofluorescein diacetate (DCHF-DA) was used as fluorescent probe.The fluorescence from cells was observed under the laser confocal microscope. Time series scan of conforcal microscope was used to observe the changes of fluorescence by GLPP in mice peritoneal macrophages over time.RESULTS: The results of confocal microscopy showed that GLPP (100 mg·kg^-1, ig for 5 d) lowed fluorescence in the mice macrophages injured by alloxan (75 mg·kg^-1 iv).GLPP (10 mg·L^-1)also lowed fluorescence in the mice macrophages injuredby tBOOH (7.76 ×10^-5mol·L^-1) in vitro as well. Time series scan showed that GLPP (10 mg·L^-1) lowed fluorescence in the mice macrophages at rest state or during the respiratory burst induced by PMA (50 nmol·L^-1).CONCLUSION: GLPP shows antioxidant effects and might have free radical scavenging effects on peritoneal macrophages in mice.