| 白藜芦醇诱导K562 细胞凋亡过程中活性氧水平的改变 |
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| 引用本文： | 郝春燕,苏海翔,魏虎来,张哲文.白藜芦醇诱导K562 细胞凋亡过程中活性氧水平的改变[J].金属学报,2004,9(10):1147-1149. |
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| 作者姓名： | 郝春燕 苏海翔 魏虎来 张哲文 |
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| 作者单位： | 兰州医学院生物化学与分子生物学教研室, 1医学实验中心, 兰州730000, 甘肃 |
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| 摘 要： | 目的: 探讨白藜芦醇对K562 细胞的凋亡诱导效应和对K562 细胞内活性氧水平的影响。方法: 应用噻唑蓝(MTT) 比色法、光镜、DNA 琼脂糖凝胶电泳和流式细胞术(FCM) 检测细胞凋亡;FCM 测定细胞内活性氧(ROS) 水平。结果: 白藜芦醇显著抑制K562 细胞增殖, 并呈现典型的凋亡形态学改变;DNA 电泳可见梯状条带出现;FCM 分析显示S 期细胞数明显增多, 出现S/G2 期阻滞, ROS 水平升高。结论: 白藜芦醇诱导K562 细胞凋亡与细胞内活性氧水平升高有关。
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| 关 键 词： | 白藜芦醇 白血病 K562 细胞凋亡 活性氧 |
| 收稿时间： | 2004-07-31 |
| 修稿时间： | 2004-09-02 |
Changes of reactive oxygen species in apoptosis induced by resveratrol in K562 cells |
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| HAO Chun-Yan,SU Hai-Xiang,WEI Hu-Lai,ZHANG Zhe-Wen.Changes of reactive oxygen species in apoptosis induced by resveratrol in K562 cells[J].Acta Metallurgica Sinica,2004,9(10):1147-1149. |
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| Authors: | HAO Chun-Yan SU Hai-Xiang WEI Hu-Lai ZHANG Zhe-Wen |
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| Affiliation: | Department of Biochemistry and Molecular Biology, 1Laboratory Center for Medical Science, Lanzhou Medical College, Lanzhou 730000, Gansu, China |
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| Abstract: | AIM: To investigate the apoptosis of K562 cells induced by resveratrol and the influence of intracellular reactive oxygen species (ROS) level.METHODS: MTT assay, light microscopy, DNA agarose gel electrophoresis and cell cycle analysis were used for examine apoptosis in K562 cells. Intracellular ROS level was measured with flow cytometry (FCM).RESULTS: Resveratrol inhibited growth of K562 cells. Agarose gel electrophoresis showed evident DNA fragmentation. Cell cycle analysis indicated increased S phase proportion, as well as apparent S/G2 phase arrest. After application of resveratrol, the level of the markedly increased of ROS.CONCLUSION: Resveratrol induces apoptosis in K562 cells may be through stimulating ROS production. |
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| Keywords: | resveratrol leukemia K562 apoptosis reactive oxygen species (ROS) |
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