竹叶提取液对小鼠的抗氧化作用

目的 探讨竹叶的水溶性提取液对小鼠的抗氧化及体外清除羟自由基(·OH^-) 的作用。 方法 7月龄小鼠给予竹叶提取液(8, 12 g·kg^-1, ig), 连续30 d 后, 采用生化方法测定小鼠全血的超氧化歧化酶(SOD) 和谷胱甘肽氧物酶(GSH-Px) 活性, 并检测小鼠心、肝、脑组织中脂褐质(LF)、丙二醛(MDA)和SOD 含量。采用体外抗坏血酸体系产生·OH^-的原理, 观察竹叶提取液清除·OH^-的作用。 结果 给予竹叶提取液的小鼠心、肝、脑组织和全血中SOD及GSH-Px 活力显著提高, 而MDA 和LF 含量则明显下降。竹叶提取液亦能清除抗坏血酸体系产生的·OH^-, 且·OH^-的清除率呈明显的量效相关性(r=0.976)。 结论 竹叶提取液具有直接清除机体·OH^-, 且能提高组织中抗氧化酶的活性, 抑制自由基损伤, 防止脂质过氧化的作用。

Antioxidant effects of extract solution of bamboo leaves in mice

AIM: To study the antioxidant effects of extract solution of bamboo leaves in mice and the effects of scavenging hydroxyl free radical (·OH-) in vitro. METHODS: The mice were treated with extract solution of bamboo leaves (8, 12 g·kg^-1, ig) daily for 30 d.The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in blood and the contents of lipofuscin (LF), malondialdehyde (MDA) and SOD in liver, and brain and heart by biochemical methods were determined.·OH-was induced by ascorbate-Cu 2+reaction system, and the effect of extract solution of bamboo leaves on scavenging ·OH-was estimated by colorimetry. RESULTS: The extract solution of bamboo leaves could significantly increase the activities of SOD and GSH-Px in blood and the activities of SOD in liver, brain and heart in mice, and obviously reduce the contents of LF and MDA in mice tissues.The extract solution of bamboo leaves of 0.05-1.0 g·L^-1 had markedly effects on scavenging ·OH-from 7.8 % to 87.7 % in dose-effect relationship (r=0.976). CONCLUSION: The extract solution of bamboo leaves can obviously inhibit damage of free radical and prevent lipid peroxidation.The mechanisms may associate with directly scavenging ·OH-, and with increasing the activities of antioxidation enzymes in mice tissues.