丹参预防自发性高血压大鼠左室肥厚及其对c-fos 表达的影响

目的: 观察丹参预防自发性高血压大鼠左室肥厚的作用,并研究其对心肌c-fos 的影响。方法: 18 只8 周龄的自发性高血压大鼠随机分成3 组,每组6 只。对照组于8 周处死,丹参组及高血压组分别经腹腔注射丹参或蒸馏水(1 g·kg^-1·d^-1),共10周。测量大鼠尾动脉收缩压(SBP)及左心室重量指数(LVMI)。应用HE 和VG 染色、免疫组织化学的方法,结合计算机图像分析技术,检测心肌细胞的直径和面积、心肌组织胶原体积比例(CVF)、血管周围胶原面积和管腔面积比例(PVCA)以及c-fos 表达。结果: 与8 周龄的自发性高血压大鼠相比,18 周龄大鼠的SBP、LVMI、心肌细胞的直径、面积、CVF、PVCA显著增加,c-fos 表达明显,丹参治疗可抑制LVH的发展和心肌组织c-fos 的表达,但对收缩压无明显改变。结论: 长期应用丹参治疗可预防自发性高血压大鼠左室肥厚的形成,其机制可能与丹参降低了心肌细胞c-fos 的表达有关。

Effects of Salvia miltiorrhiza Bge on left ventricular hypertrophy in spontaneously hypertensive rats and expression of c-fos

AIM: To observe the effects of Salvia miltiorrhiza Bge (SMB) on left ventricular hypertrophy (LVH) in spontaneously hypertensive rats (SHR) and the expression of c-fos.METHODS: 18 SHRs in 8 weeks old were divided into three groups at random.SMB or distilled water(1 g·kg^-1 ·d^-1) was injected intraperitoneally to two groups for 10 weeks.Systolic blood pressure(SBP) and left ventricular mass index(LVMI) were measured.HE, VG and immunohistochemical staining combined with computed morphometry were used to evaluated the cardiomyocyte size and diameter, the collagen volume fraction(CVF), perivascular circumferential area (PVCA) and c-fos expression in the left ventricular tissue.RESULTS: Compared with 8-week-old rats, the SBP, LVMI, cardiomyocyte size and diameter, CVF, PCVA, c-fos expression increased markedly in the 18th week of SHRs.The LVH stopped and c-fos expression decreased whereas SBP changed slightly in animals treated with SMB.CONCLUSION: Chronic treatment with SMB can inhibit the development of LVH in SHR, which is probably related to the decease of cardiac c-fos.