逍遥滴鼻液对实验性偏头痛模型大鼠c-fos 基因表达的影响

目的: 研究逍遥滴鼻液通过鼻腔给药对实验性偏头痛模型大鼠c-fos 基因表达的影响,探讨逍遥滴鼻液治疗偏头痛的作用机理。方法: 将60只Wistar大鼠随机分为6 组,即正常对照组、模型组、生理盐水滴鼻组、太极通天口服液组、逍遥滴鼻液灌胃组及逍遥滴鼻液滴鼻组,根据文献方法改进,用硝酸甘油9.5mg·kg^-1剂量皮下注射复制偏头痛模型,用免疫组化SABC 法观察c-fos 基因表达的阳性细胞数、面积及灰度,并进行组间比较。结果: 逍遥滴鼻液滴鼻组大鼠脑干、下丘脑中的c-fos 表达阳性细胞数目和面积显著低于逍遥滴鼻液灌胃组和太极通天口服液灌胃组。结论: 逍遥滴鼻液能显著抑制c-fos的表达,其作用机理可能是干预c-fos产生的某个环节,在作用效果上优于太极通天口服液,鼻腔给药优于灌胃给药。

Expression of c-fos in xiaoyao nose dribbling liquid on experimental migrainous rat models

AIM: To probe the mechanism of the xiaoyao nose dribbling liquid (XNDL) by investigating the expression of c-fos in experimental migrainous rat models.METHODS: Sixty Wistar rats were divided into normal control, model group, saline nose dripped group, taijitongtian po group, XNDL po group and XNDL nose dripped group at random, and the experimental migrainous models were duplicated by subcutaneous injection of nitroglycerin 9.5 mg·kg^-1.The positive cell numbers, area and gray scale were observed by the immunohistochemical method.RESULTS: The positive cell numbers and area in brain stem and hypothalamus of XNDL nose dripped group were significantly less than that of XNDL and taijitongtian po groups.CONCLUSION: XNDL may significantly inhibit the expression of c-fos.Its mechanism may be related to stop certain link of c-fos productions.