中华眼镜蛇毒F组分对血管平滑肌细胞增殖的影响

目的 研究中华眼镜蛇毒F 组分对血管平滑肌细胞增殖的影响。 方法 用体外培养的血管平滑肌细胞, 观察F 组分对20 %小牛血清引起的细胞对氚标胸腺嘧啶核苷酸(H³-TdR) 掺入的影响, 用蛋白质免疫印迹法(Western blotting) 测定F 组分对20 %小牛血清引起的细胞内原癌基因、核增殖抗原(Proliferationcell nuclear antigen, PCNA) 表达的影响。 结果 F 组分呈浓度依赖性抑制20 %小牛血清引起的细胞对H³-TdR 掺入率和细胞内C-myc、PCNA 的表达, 其中30、100 mg·L^-1给药组与对照组比较有显著差异(P <0.05)。 结论 中华眼镜蛇毒F 组分通过影响细胞内原癌基因C-myc、PCNA 的表达, 抑制血管平滑肌细胞的增殖。

Effect of fraction F of Naja naja atra venom on proliferation of vascular smooth muscle cells

AIM: To study the effect of fraction F of Naja naja atra venom on proliferation of vascular smooth muscle cells (VSMC). METHODS: The rate of incorporation of [ ³H] thymidine (³H-TdR) into VSMC was observed by cultured VSMC in vitro, and expression of oncogene C-myc and proliferation cell nuclear antigen (PCNA) in VSMC stimulated by 20 % serum was assayed by protein immunoblotting (western blotting). RESULTS: Fration F significantly inhibited the incorporation of³HTdR into VSMCs and the expression of oncogene C-myc and PCNA in VSMC which was stimulated by 20 %serum in a dose-dependent manner.Groups of 30 and 100 mg·L^-1 had obvious difference (P <0.05) when compared with control group. CONCLUSION: Fraction F of Naja naja atra venom inhibits the proliferation of VSMC by affecting expression of oncogene C-myc and PCNA.