益智胶囊对大鼠脑弥散性缺血再灌注后海马CA1 区神经元迟发性死亡的影响

目的 观察益智胶囊对大鼠全脑缺血后海马CA1 区神经元迟发性死亡及学习功能的影响。 方法 使用四血管闭塞(4-VO) 法制成大鼠急性全脑缺血再灌注模型。采用免疫组织化学方法计数脑缺血再灌注脑损伤后1、2、4、8 及40 d 后, 大鼠海马CA1区正常神经元数量;并用Morriss 水迷宫观察脑缺血再灌注脑损伤后40 d 时各组大鼠的学习记忆功能。 结果 从缺血再灌注后d 2 起, 益智胶囊(100 mg·kg^-1) 组大鼠海马CA₁ 区正常神经元数量明显多于缺血对照组大鼠海马CA₁ 区正常神经元数量, 差异有明显统计学意义(P <0.01)。同时,Morris 水迷宫法检测全脑缺血再灌注脑损伤后40 d时各组大鼠记忆功能, 益智胶囊(100 mg·kg^-1) 组大鼠明显好于缺血对照组大鼠(P <0.01)。 结论 益智胶囊对全脑缺血后大鼠海马CA1 区神经元具有保护作用, 并可改善大鼠的记忆功能障碍。

Effects of Capsule Yi-Zhi on delayed neuronal death in hippocampal CA1 region and memory function after global cerebral ischemia in rats

AIM: To observe the effects of Capsule Yi-Zhi on the delayed neuronal death in hippocampal CA₁ region and memory function after global cerebral ischemia. METHODS: The model of acute reperfusion injury after cerebral ischemia in rats was made by vertebral and carotid arteries occlusion;after the models were made 1, 2, 4, 8 and 40 d, the neurons in hippocampal CA₁ region were immunohistochemically stained and calculated.The Morris water maze test was carried out to observe the learning and memory capacities in the 40th day. RESULTS: The number of normal neurons was significantly higher in the Capsule Yi-Zhi (100 mg·kg^-1) group than that of the control group.Compared with the control group, the rats in the Capsule Yi-Zhi (100 mg·kg^-1) group decreased time in finding the platform under the water surface in Morris water maze test. CONCLUSION: CYZ has protective effects on neurons in hippocampal CA₁ region, and shows promising effects on learning and memory dysfunction after global ischemia / reperfusion injury.