葛根素对β-淀粉样肽所致痴呆模型小鼠学习记忆的影响

目的 研究葛根素对β-淀粉样肽所致痴呆模型小鼠学习记忆障碍的影响。 方法 以β-淀粉样肽3 μl(1 mmol·L^-1) icv 制备小鼠学习记忆障碍模型,用Morris 水迷宫法检测小鼠学习记忆能力。同时检测脑中及血中超氧化歧化酶(SOD)、丙二酰醛(MDA) 含量。 结果 葛根素25 和50 mg·kg^-1均能改善模型小鼠的学习记忆(P <0.05 或P <0.01)。生化指标测定显示葛根素能使模型小鼠脑及血中的SOD 增多、MDA 减少(P <0.05 或P <0.01)。 结论 葛根素可对抗β-淀粉样肽的神经毒性, 其机制可能与清除脑自由基及提高抗氧化活性作用有关。

Effects of puerarin on AD animal model in mice induced by β-amyloid peptide

AIM: To observe the effects of puerarin on leaning and memorizing in mice induced by β-amyloid peptide. METHODS: β-amyloid peptide (25-35, 3 μl, 1.0mmol·L^-1) icv to mice was used as an AD animal model.Learning and memorizing ability inmice were inspected through Morris water maze test.In the meantime, the content of SOD and MDA in the mice of cerebral tissue and blood were also inspected. RESULTS: Puerarin 25 and 50mg·kg^-1 improved learning andmemorizing ability in model mice (P <0.05 or P <0.01). And puerarin increased the content of SOD and reduce the content of MDA in cerebral tissue and blood of model mice. CONCLUSION: Puerarin can resist nerve poison of β-amyloid peptide, and the mechanism may be relevant to purge brain free radicals and to increase the activities of antioxidase.