复方沙棘颗粒对大鼠皮层神经细胞缺血性损伤的保护作用

目的: 研究复方沙棘颗粒对体外培养的大鼠大脑皮层神经细胞谷氨酸(Glu) 损伤的保护作用及机理。方法: 分离培养新生(1 d) 大鼠大脑皮层神经细胞, 加入Glu 模拟兴奋性损伤,MTT 法测定细胞存活率, 比色法测定上清中乳酸脱氢酶(LDH) 含量, 细胞中超氧化物歧化酶(SOD) 、谷胱甘肽(GSH-Px) 活性、丙二醛(MDA) 含量。结果: 细胞经Glu 损伤后,上清LDH 含量、细胞中MDA 含量显著增加, SOD 和GSH-Px 活力明显下降。复方沙棘处理组可有效防止上述改变。结论: 复方沙棘颗粒可有效保护由Glu 引起的大鼠大脑皮层神经细胞的损伤。其机理可能与抗脂质过氧化、清除自由基有关。

Protection of compound Hippophae rhamnoids L-granule on ischemic injury in cultured rat cortical neurons

AIM: To study the protection of compound Hippophae rhamnoides L-granule (CHRG) on ischemic injury induced by glutamic acid (Glu) in the cortical neurons of rat cultured in vitro and its relevant mechanism .METHODS: The cortical neurons of the newborn rats (1 d) were cultured and separated.The protective effects of CHRG on ischemic injury induced by Glu were observed.The survival rate of the neurons was measured by MTT method, the content of LDH in extra-cellular bathing media was measured by colorimetry as well as the content of MDA, the activity of SOD and GSH-Px in the homogenate of cortical cells.RESULTS: When cells were treated with Glu, the content of LDH and MDA increased and the activity of SOD and GSH-Px decreased significantly.The observed group treated with CHRG could effectively prevent these changes.CONCLUSION: The serum preparation of CHRG shows protective effects on the cortical neurons injury induced by Glu and the mechanism may be related to suppressing the generation of lipids peroxides and cleaning excessive free radicals.