霉酚酸酯对2 型糖尿病大鼠肾脏的保护作用

目的: 探讨霉酚酸酯对2 型糖尿病大鼠肾脏的保护作用及其机制。方法: 将实验动物分为正常对照组、糖尿病组及霉酚酸酯治疗组。霉酚酸酯组给予霉酚酸酯(15 mg·kg^-1·d^-1) 治疗。13 周后检测各组大鼠尿白蛋白排泄率、内生肌酐清除率、血糖、血脂、血胰岛素;HE 及PAS 染色观察肾脏病理改变;免疫组化方法检测肾组织中结缔组织生长因子(CTGF) 及胶原(Col I 、Col Ⅲ) 的表达。结果: 霉酚酸酯可降低尿白蛋白排泄率、内生肌酐清除率、减轻早期肾小球肥大, 且不同程度降低肾组织CTGF 表达及Col I 、Col Ⅲ的沉积。结论: 霉酚酸酯对2 型糖尿病大鼠肾脏有保护作用, 此作用可能与霉酚酸酯降低肾组织中CTGF 的表达, 减少系膜外基质的沉积有关。

Protective effects of mycophenolate mofetil on kidneys of type 2 diabetic rats

AIM: To evaluate the protective effects of mycophenolate mofetil on the kidneys of type 2 diabetic rats and discover their mechanisms.METHODS: Wistar rats were divided into three groups, such as normal control rats, diabetic rats, and diabetic rats in the treatment with mycophenolate mofetil (MMF, 15 mg·kg^-1·d^-1). Thirteen weeks later, urinary albumin excretory rate (UAE), creatine clearance (Ccr), blood glucose, blood insulin and blood lipid were measured, and kidney pathology was observed. Inmmunohistochemistry was used to analyze the expression of CTGF, ColI and Col Ⅲ.RESULTS: Mycophenolate mofetil decreased UAE, Ccr and reduced glomerular volume. The expression of CTGF and deposition of ECM decreased after diabetic rats received mycophenolate mofetil.CONCLUSION: Mycophenolate mofetil can protect the kidney of diabetic rats. Its mechanism may be related to the decrease of CTGF expression and extracellular matrix deposition in renal tissue.