阿魏酸乙酯对过氧化氢损伤人血管内皮细胞的保护作用

目的: 观察阿魏酸乙酯对人血管内皮细胞的保护作用。方法: 实验分对照组、模型组、阿魏酸4个剂量组、阿魏酸乙酯4个剂量组。过氧化氢诱导血管内皮细胞损伤,用四甲基偶氮唑蓝比色法检测细胞存活数量,用比色法测细胞培养液中过氧化物丙二醛含量及乳酸脱氢酶的活性。结果: 阿魏酸乙酯及阿魏酸不同剂量(2,4,8,16 nmol·L^-1 )对过氧化氢损伤的人血管内皮细胞具有保护作用,可抑制过氧化氢损伤的血管内皮细胞释放乳酸脱氢酶和丙二醛。相同剂量组的阿魏酸乙酯的作用强于阿魏酸。结论: 阿魏酸乙酯具有减轻过氧化氢损伤,保护血管内皮细胞的作用,并且其作用强于阿魏酸。

Protective effects of ethyl ferulate on injured vascular endothelial cells (EC) by H2O2

AIM: To observe protective effects of ethyl ferulate (EF) on injured vascular endothelial cells (VECs) by hydrogen peroxide (H₂O₂).METHODS: Experimental groups were set as control group, H₂O₂group, ethyl ferulate group and ferulic acid (AF) group.The proliferation of vascular endothelial cells was assessed by the methyl thiazolyl tetrazolium (MTT) assay.Malonaldehyde (MDA) and lactate dehyolrogenase (LDH) were determined to evaluate lipid peroxidation of VECs by the spectro photometric assay.RESULTS: Ethyl ferulate and ferulic acid at concentrations of 2, 4, 8, and 16 nmol·L^-1 could protect vascular endothelial cells from the injure by H₂O₂ and reduce LDH and MDA production.CONCLUSION: Ethyl ferulate exerts the protective effects on injured vascular endothelial cells by H2O2.The activity of EF is higher than that of AF.Its mechanism may be related to antioxidant effect of lipids peroxidation.