川芎嗪联用左旋精氨酸对缺血-再灌注损伤兔肝脏能量代谢的影响

目的: 探讨川芎嗪(LGT)、左旋精氨酸（L-Arg)联合使用对肝缺血-再灌注损伤（HIRI)时肝细胞能量代谢的影响及其机制。方法: 实验兔40只,建立肝缺血-再灌注模型后随机分4组:模型组,LGT组,L-Arg组和LGT + L-Arg组,每组10只。再灌注45 min时,分别检测肝组织内三磷酸腺苷（ATP)、二磷酸腺苷（ADP)、一磷酸腺普（AMP)含量、总腺苷酸量(TAN)、能荷（EC)、丙二醛浓度（MDA)、超氧化物歧化酶活性（SOD)、一氧化氮代谢产物（N0₂^-/N0₃^-)水平、血栓素B₂(TXB₂)、6-酮基-前列腺素F_1α/6-keto-PGF_1α含量和 TXB₂/6-keto-PGF_1α比值。结果: 与模型组比较,LGT组、L-Arg组、LGT + L-Arg组肝组织内 ATP、EC、NO₂^-/NO₃^-、6-keto-PGF_1α含量及SOD活性均明显增高（P<0.05和P<0.01),MDA、TXB₂含量及TXB₂-keto-PGF_1α比值均显著减少（P<0.05和P<0.01)。结论: LGT联用L-Arg可通过降低体内氧自由基水平、提高一氧化氮水平、糾正TXA₂与PGI₂的平衡,而改善缺血-再灌注损伤肝脏的能量代谢。

Effects of ligustrazini and L-arginine on hepatocellular energy metabolism in rabbits with reperfusion injury after ischemia

AIM: To study the effects of ligustrazini (LGT) and L-arginine (L-Arg) on hepatocellular energy metabolism in the rabbits with reperfusion injury after he-patic ischemia and its mechanisms. METHODS: 40 rab-bits were randomly divided into 4 groups (n=10 in each), hepatic ischemia-reperfusion (HIR) group, HIR + LGT group (B), HIR + L-Arg group (C) and HIR + LGT + L-Arg group (D). The contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), ad-enosine monophosphate (AMP),total adenylic acid num-ber (TAN), energy charge (EC), malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide prod-ucts (N0₂^-/N0₃^-), thromboxane B₂ (TXB₂), and 6-ke-to-prostaglandin F_1α (6-keto-PGF_1α) ratio in the liver tis-sue were measured at 45 minutes after reperfusion. RESULTS: The contents of ATP, NO, 6-keto-PGF_1α and SOD activity of the liver tissue in B, C, and D groups were higher than those in HIR group (P < 0.05 or P < 0.01), the contents of MDA, TXB₂ and TXB₂/6-keto-PGF_1α ratio of the liver tissue in B,C, and D groups were lower than those in HIR group (P<0.05 or P<0.01). CONCLUSION: The combination usage of LGT and L-Arg can improve the hepatocellular energy metabolism in the reperfusion injury after liver ischemia by decreasing oxygen free radical level, raising NO level and correcting TKA₂/PGI₂ imbalance.