Separation and identification of isomeric conjugated fatty acids by high-performance liquid chromatography with photodiode array detection |
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Authors: | Sajid Husain K Sita Devi |
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Affiliation: | (1) Analytical Division, Indian Institute of Chemical Technology, (CSIR), 500 007 Hyderabad, India |
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Abstract: | A simple, high-performance liquid chromatographic method is described for the separation of tetraenoic, trienoic and dienoic
conjugated fatty acids on a Zorbax ODS reversed-phase column using acetonitrile/tetrahydrofuran (95∶5, vol/vol) at a flow
rate of 1.2 mL/min as mobile phase. Also described is the separation of the isomeric conjugated fatty acids with acetonitrile/water/tetrahydrofuran
(90∶90∶1, by vol) as mobile phase. The simultaneous detection and identification of the separated geometrical isomers in the
eluant was accomplished using photodiode array detection. |
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Keywords: | |
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