好氧丰盛/缺氧饥饿模式驯化微生物积累内源聚合物并实现内源反硝化

为了探究好氧丰盛/缺氧饥饿（O/A-F/F）模式驯化的微生物的内源积累能力和缺氧段内源反硝化的特性，并比较其与传统好氧丰盛/饥饿（O-F/F）驯化模式的差别，以添加乙酸的生活污水为底物，在F/F=0.1的条件下，采用O/A-F/F和O-F/F两种不同模式驯化活性污泥.结果表明，O/A-F/F模式的PHA产率为0.52，O-F/F模式的PHA产率为0.78.虽然O/A-F/F模式的PHA产率低，但是O/A-F/F模式具有内源反硝化功能，比内源反硝化速率为2.76 mg/（h·g）.O/A-F/F模式下还原1 mol的硝酸盐需要2 mol的内源PHA.2种选择模式下的菌群结构呈现差异，O/A-F/F模式下优势菌群为Betaproteobacteria、Unclassified Bacteroidetes、Lgnavibacteria和Bacteroidia.O-F/F模式富集的微生物主要为Betaproteobacteria、Flavobacteria、Alphaproteobacteria和Gammaproteobacteria.O/A-F/F运行模式可以实现好氧阶段污水中有机物的内源储存并在缺氧段用于反硝化.

Biotechnological production of polyhydroxyalkanoates:a review on trends and latest developments

To explore the ability of accumulation of endogenous polymers and denitrification characteristics driven by endogenous polymers of microorganisms acclimated by aerobic/anoxic F/F (O/A-F/F) mode, and compare with traditional aerobic F/F (O-F/F) mode, sewage wastewater added with acetate as carbon source was used in this study. The F/F=0.1 was applied to acclimated activated sludge by O/A-F/F and O-F/F modes. The abilities of acclimated microorganisms under two modes were compared. Results show that the PHA yield of O/A-F/F and O-F/F modes is 0.52 and 0.78, respectively. Although the PHA yield under O/A-F/F mode is lower, the function of denitrification driven by PHA can be achieved. The specific denitrification rate is 2.76 mg/(h·g). The denitrification stoichiometric coefficient is 2.0 mol PHA per mol NO₃^--N. Betaproteobacteria, unclassified Bacteroidetes, lgnavibacteria and Bacteroidia are dominant microorganisms after selection by O/A-F/F mode while Betaproteobacteria, Flavobacteria, Alphaproteobacteria and Gammaproteobacteria are dominant microorganisms under O/-F/F mode. The organic matter can be stored as endogenous polymers under O/A-F/F mode during aerobic and be applied to driving denitrification during anoxic achieved.