| 麦冬多糖OPF-1的结构分析 |
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| 引用本文： | 吕宁,浦军平,金凤燮,鱼红闪. 麦冬多糖OPF-1的结构分析[J]. 大连工业大学学报, 2012, 31(4): 243-246 |
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| 作者姓名： | 吕宁 浦军平 金凤燮 鱼红闪 |
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| 作者单位： | 1. 大连工业大学生物工程学院,辽宁大连,116034
2. 张家港市华昌药业有限公司,江苏张家港,215600 |
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| 基金项目： | 国家自然科学基金资助项目，辽宁省高等学校优秀人才支持计划项目，辽宁省科学技术基金 |
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| 摘 要： | 分离纯化麦冬多糖,得到多糖组分OPF-1,并对其一级结构进行初步分析。水提醇沉法得到麦冬粗多糖,Sevage法除蛋白后经Sephadex G-100柱分离纯化得到OPF-1。运用完全酸水解和HPLC法测定麦冬多糖OPF-1的单糖组成;并采用高碘酸氧化、Smith降解、GC、IR等方法研究OPF-1的化学结构。结果表明,麦冬多糖OPF-1为果聚糖,其相对分子质量为48000,主要由2→1和2→6糖苷键连接的呋喃型果糖组成。该多糖中还含有少量的葡萄糖(果糖与葡萄糖的摩尔比为16∶1),可能连接在多糖的还原性末端。
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| 关 键 词： | 麦冬多糖 结构表征 链接方式 |
Structural analysis of a polysaccharide OPF-1 from Ophiopogon japonicus |
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| LV Ning , PU Jun-ping , JIN Feng-xie , YU Hong-shan. Structural analysis of a polysaccharide OPF-1 from Ophiopogon japonicus[J]. Journal of Dalian Dalian Polytechnic University, 2012, 31(4): 243-246 |
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| Authors: | LV Ning PU Jun-ping JIN Feng-xie YU Hong-shan |
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| Affiliation: | 1.School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China;2.Zhangjiagang Huachang Pharmaceutical Company Limited,Zhangjiagang 215600,Chin |
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| Abstract: | The structure of a polysaccharide OPF-1 obtained from the Ophiopogon japonicus polysaccharide was analized.OPF-1 was obtained using sephadex G-100 column,following water extract of Ophiopogon japonicus was precipitated by ethanol and deproteinized by method of Sevage.The chemical structure of OPF-1 was analized using methods of complete hydrolysis,HPLC,periodate oxidation,Smith degradation,GC and IR et al.The results indicate that the relative molecular mass of OPF-1 was estimated to be 48 000,which was mainly composed of Fruf by linkage of 2→1 or 2→6.The polysaccharide OPF-1 contains trace glucoses [n(fructose)∶n(glucose)=16∶1],which maybe connected to its reducing teminal. |
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| Keywords: | Ophiopogon japonicus polysaccharide structure characterization link mode |
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