| 绞股蓝皂苷糖苷酶的分离纯化及酶性质的研究 |
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| 引用本文： | 毛泽仁,张景,金凤燮,鱼红闪.绞股蓝皂苷糖苷酶的分离纯化及酶性质的研究[J].大连工业大学学报,2012,31(2):79-82. |
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| 作者姓名： | 毛泽仁 张景 金凤燮 鱼红闪 |
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| 作者单位： | 1. 大连工业大学生物工程学院,辽宁大连,116034
2. 大连市产品质量监督检验所,辽宁大连,116021 |
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| 基金项目： | 国家自然科学基金资助项目(30371744);辽宁省科学技术基金资助项目(20042132);辽宁省教育厅创新团队项目(2009T007) |
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| 摘 要： | 为得到纯的绞股蓝皂苷糖苷酶,对Absidiasp.GYP4r菌所产的酶进行了分离提纯,并对其酶反应条件进行优化。该酶经75%饱和度的硫酸铵沉淀、DEAE-cellulose DE52阴离子交换柱分离、SDS-聚丙烯酰胺凝胶电泳提纯,得到纯酶,分子质量约为68ku。酶学性质研究表明,酶反应的最适温度为40℃,在20～60℃稳定,最适pH为5.0,在pH 2.2～8.0稳定。Cu2+对该酶的活性有一定的抑制作用,Na+、K+、Mg2+、Zn2+、Fe3+、Ca2+对酶的活性没有影响。该酶的米氏常数为14.20mmol/L,最大反应速率为0.46mmol/(L.h)。
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| 关 键 词： | 绞股蓝皂苷 绞股蓝皂苷糖苷酶 酶反应 |
Purification and characterization of gypenosidase |
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| MAO Ze-ren
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ZHANG Jing
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JIN Feng-xie
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YU Hong-shan.Purification and characterization of gypenosidase[J].Journal of Dalian Dalian Polytechnic University,2012,31(2):79-82. |
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| Authors: | MAO Ze-ren ZHANG Jing JIN Feng-xie YU Hong-shan |
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| Affiliation: | 1.School of Biological Engineering,Dalian Polytechnical University,Dalian 116034,China; 2.Dalian Institute of Product Quality Supervision Inspection,Dalian 116021,China |
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| Abstract: | Gypenosidase was purified and charaterized from Absidia sp.GYP4r.The enzyme was capable of hydrolyzing some glycosyl of Gypenosides to produce new Gypenosides containning lower sugar.The SDS-PAGE analysis showed that the molecular weight of the enzyme was about 68ku.The optimum pH and temperature were 5.0 and 40 ℃,the enzyme was stable at 20-60 ℃ and pH 2.2-8.0 respectively.Its Km value was 14.20 mmol/L and the maximum rate was 0.46 mmol/(L·h).Na+,K+,Mg2+,Zn2+,Fe3+,Ca2+ showed no effects on the enzyme activity,while Cu2+ could inhibit it to a certain extend. |
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| Keywords: | gypenoside gypenoside-glycosidase enzyme reaction |
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