| 仿刺参体表微生物的菌相分析 |
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| 引用本文： | 成功,裴赢莹,张公亮,侯红漫. 仿刺参体表微生物的菌相分析[J]. 大连工业大学学报, 2012, 31(2): 91-94 |
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| 作者姓名： | 成功 裴赢莹 张公亮 侯红漫 |
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| 作者单位： | 大连工业大学食品学院,辽宁大连,116034 |
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| 摘 要： | 采用3种培养基对大连仿刺参体表微生物进行分离,并通过细菌形态、生理生化特性和16SrD-NA分子生物学相结合的方法对分离纯化的细菌进行鉴定,从而了解大连仿刺参体表的菌群组成。结果表明,从仿刺参体表分离纯化了105株细菌,鉴定出10种不同的菌属分别为交替假单胞菌属(Pseud-oalteromonas)、希瓦氏菌属(Shewanella)、假单胞菌属(Pseudomonas)、弧菌属(Vibrio)、枯草芽孢杆菌(Bacillus subtilis),变形菌属(Proteobacterium)、Alteromonadacea、不动杆菌属(Acinetobacter)、解淀粉芽孢杆菌(Bacillus amyloiquefaciens),腐生葡萄球菌(Staphylococcus saprophyticus),其中交替假单胞菌属和弧菌属分别占分离菌株的23.8%和22.9%。
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| 关 键 词： | 仿刺参 分离鉴定 细菌菌相分析 |
Analysis of bacterial flora on Apostichopus Japonicus surface |
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| CHENG Gong , PEI Ying-ying , ZHANG Gong-liang , HOU Hong-man. Analysis of bacterial flora on Apostichopus Japonicus surface[J]. Journal of Dalian Dalian Polytechnic University, 2012, 31(2): 91-94 |
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| Authors: | CHENG Gong PEI Ying-ying ZHANG Gong-liang HOU Hong-man |
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| Affiliation: | School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China |
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| Abstract: | The microorganisms on the surface of Dalian Apostichopus japonicas were isolated with three kinds of media.The isolated and purified microorganisms were identified based on bacterial morphs,physiological-biochemical characteristic and 16S rDNA molecular biology to find out the bacterial composition of microflora on the surface of Dalian sea-cucumber.The result showed that 105 bacteria were identified and purified from the surface of sea cucumber.Among them 10 different kinds of genera were identified as Pseudoalteromonas,Shewanella,Pseudomonas,Vibrio,Bacillus subtilis,Proteobacterium,Alteromonadaceas,Acinetobacter,Bacillus amyloiquefaciens,Staphylococcus saprophyticus,of which Pseudoalteromonas and Vibrio accounted for 23.8% and 22.9% respectively. |
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| Keywords: | Apostichopus japonicus isolation and identification bacteria flora analysis |
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