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| 5-氨基乙酰丙酸脱水酶缺失对大肠杆菌生长的影响 | |
| 引用本文： | 尚柯,郭小飞,王艳萍,郑平.5-氨基乙酰丙酸脱水酶缺失对大肠杆菌生长的影响[J].现代食品科技,2011,27(7):742-746. |
| 作者姓名： | 尚柯 郭小飞 王艳萍 郑平 |
| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津,300457;中科院天津工业生物技术研究所,天津,300308 2. 中科院天津工业生物技术研究所,天津,300308 3. 天津科技大学食品工程与生物技术学院,天津,300457 |
| 基金项目： | 国家自然科学基金，天津科委应用基础与前沿技术研究计划基金，中科院优秀青年科技专项基金 |
| 摘 要： | 5-氨基乙酰丙酸(ALA)脱水酶直接关系到ALA的积累,研究该酶突变对细胞的影响对生物法生产ALA具有重要意义,然而目前这方面认识比较混乱.本研究通过无痕敲除Ecoli MG1655菌株的ALA脱水酶的结构基因hemB,发现ALA脱水酶酶活大幅降低,且突变株生长受到极大的影响,但是仍然有10%的残余酶活支持细胞微弱且不... |
| 关 键 词： | 5-氨基乙酰丙酸 ALA脱水酶 无痕敲除 |
Influence of 5-Aminolevulinic Acid Dehydratase Deletion on E.coli Growth |
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| SHANG Ke,GUO Xiao-fei,WANG Yan-ping,ZHENG Ping.Influence of 5-Aminolevulinic Acid Dehydratase Deletion on E.coli Growth[J].Modern Food Science & Technology,2011,27(7):742-746. | |
| Authors: | SHANG Ke GUO Xiao-fei WANG Yan-ping ZHENG Ping |
| Affiliation: | 1.Food Engineering and Biotechnology College,Tianjin University of Science & Technology,Tianjin 300457,China)(2.Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China) |
| Abstract: | 5-Aminolevulinic acid dehydratase(ALAD) affects the accumulation of ALA.Therefore study of the effect of ALAD mutation on cells is of great significance for ALA bioproduction.However,the present knowledge is still contradictive.In this study,by knocking out ALAD-encoding gene hemB of E.coli MG1655,the enzyme activity and growth of the mutant were compared with those of the wild type strain.It was found that the ALAD activity of the mutant was dramatically decreased.The cells grew very slowly compared to the wild type.Interestingly,10% residual activity was detected and was supposed to support the weak growth of the mutant,which was not evidently resumed by the addition of ALA.The results improve the understanding of ALA metabolism and will benefit for the construction of ALA-producing strain. |
| Keywords: | 5-aminolevulinic acid(ALA) ALA dehydratase gene knockout |
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