超声强化响应面法优化知母多糖的提取工艺

利用响应面法优化超声强化提取知母多糖工艺条件.在单因素试验基础上,采用Box-Benhnken中心组合试验,以提取时间、液料比、超声功率和提取次数为影响因素,以知母多糖得率为响应值建立二次回归方程,通过响应面分析得到优化组合.在分析各个因素的显著性和交互作用后,结合实际操作得出知母多糖浸提的最佳工艺条件为:提取时间11...

Response Surface Methodology for the Optimization of Ultrasound assisted Extraction of Polysaccharides from Rhizoma Anemarrhenae

Ultrasound pretreatment was applied to improve the extraction process of polysaccharides from Rhizoma Anemarrhenae.Single factor experiments for extraction time,ratio of solvent to material,extraction times and ultrasonic power were firstly performed to investigate the effect on the yield of Polysaccharides from Rhizoma Anemarrhenae.Subsequently,response surface methodology based on Box-Behnken experimental design was employed to evaluate the interactive effects of four crucial variables including extraction time,ratio of solvent to material,extraction times and ultrasonic power.One mathematical model for the yield of Polysaccharides from Rhizoma Anemarrhenae as functions of the four variables was established.The results showed that the optimal extraction conditions of polysaccharides were as follows:extraction time 117min,ratio of solvent to material 11mL/g,extraction times 2 and ultrasonic power 92 W.Under the optimal conditions,the yield was up to 30.21%.,which was in agreement with the calculated value.