摘 要: | 目的建立反相高效液相色谱(RP-HPLC)同时测定消风止痒颗粒中梓醇、阿魏酸、毛蕊花糖苷、苍术素、升麻素苷、5-O-甲基维斯阿米醇苷、胡薄荷酮、甘草苷和甘草酸铵的方法。方法采用Waters XTERRA C_(18)色谱柱(250 mm×4.6 mm,5μm);乙腈-1.5 mmol/L KH_2PO4溶液(稀磷酸调pH3.5)(25:75)为流动相,梯度洗脱,流速1.0 ml/min;柱温:35℃,进样量:10μl。结果梓醇、阿魏酸、毛蕊花糖苷、苍术素、升麻素苷、5-O-甲基维斯阿米醇苷、胡薄荷酮、甘草苷和甘草酸铵9种成分能达到很好分离;其线性范围分别为9.076~63.53μg/ml(r=0.9995),4.660~32.62μg/ml(r=0.9997),8.452~59.16μg/ml(r=0.9993),4.028~28.19μg/ml(r=0.9996),5.088~35.62μg/ml(r=0.9994),4.992~34.94μg/ml(r=0.9991),8.020~56.14μg/ml(r=0.9992),3.792~26.55μg/ml(r=0.9993),4.116~28.81μg/ml(r=0.9991),平均加样回收率分别为99.74%,99.21%,99.54%,99.03%,99.91%,98.99%,100.04%,98.79%,99.09%,RSD分别为0.55%,0.92%,0.69%,0.81%,1.13%,0.72%,1.14%,0.59%,0.68%(n=6)。8批次消风止痒颗粒中梓醇、阿魏酸、毛蕊花糖苷、苍术素、升麻素苷、5-O-甲基维斯阿米醇苷、胡薄荷酮、甘草苷和甘草酸铵质量浓度分别为0.4101~0.4159 mg/g,0.2409~0.2448 mg/g,0.3989~0.4015 mg/g,0.1571~0.1599 mg/g,0.2557~0.2582 mg/g,0.2511~0.2551 mg/g,0.3842~0.3866 mg/g,0.1001~0.1018 mg/g,0.1251~0.1276 mg/g。结论方法简便快速、回收率高、重现性好,可用于提升消风止痒颗粒的质量标准。
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