Creation of single-particle environment by positive dielectrophoresis and liquid dielectrophoresis |
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| Authors: | Chun-Hong Chen Ming-Kun Chen Min-Haw Wang Ling-Sheng Jang |
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| Affiliation: | 1. Department of Electrical Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan
2. Department of Electrical Engineering, Chinese Culture University, Taipei, Taiwan
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| Abstract: | Two electrical mechanisms for manipulating particles and fluids, dielectrophoresis (DEP) and liquid dielectrophoresis (LDEP), are integrated in a microfluidic chip for creating the single-particle environment. The fluid is activated by LDEP with a 100-kHz/240-Vpp signal. When the single polystyrene bead approaches the trapping area, positive DEP force is utilized to capture and immobilize the bead. After trapping the bead, the process of liquid cutting and droplet creation is employed to create a droplet containing a single bead by LDEP with a 100-kHz/320-Vpp signal. |
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