花生壳中木犀草素等抑菌活性成分的提取、纯化与研究

本实验以甲醇粗提、溶剂梯度萃取、配合抑菌活性跟踪检测，得到了抑菌活性较强、富含黄酮类的花生壳乙酸乙配组分，并用酸碱沉淀、柱层析等法纯化得到三种化合物(1^#，2^#，3^#)。其中2^#从紫外光谱法确认为木犀草素，得率为0．53%。它对金黄色葡萄球菌和枯草杆菌有一定的抑制效果。实验还发现30对金黄色葡萄球菌、酿酒酵母有一定的抑菌活性；1^#则具有更广的抑菌谱，它对金黄色葡萄球菌、酿酒酵母、枯草杆菌和青霉均有较强的抑制作用。它的紫外图谱具有二氢黄酮的特点，其结构有待进一步实验认证。

Study on Extraction, Purification and Antimicrobial Activity Investigation of Luteolin and Other Components in Peanut Hulls

In this experiment the flavonoids in peanut hulls with strong antimicrobial activity were found in ethyl acetatecomponent by methanol extraction and then successive polarity-increase solvent extraction to monitor the antimicrobial effect.Through base-acid precipitation, and TLC purification three compounds (1# 2# 3#) were obtained and 2# was confirmed by UVchemical structure analysis as Luteolin (the yield was 0.53 %) to have antimicrobial activities against Staphylococcus Aurous andBacillus subtilis , while 3# to have activities against Staphylococcus Aurous and Saccharomyces. 1# should even broader antimicrobialeffects on Staphylococcus Aurous,Saccharomyces , Bacillus subtilis,and Penicilliam. According to UV, 1# might belong toflavanones, and need to be determined further.