Separation and purification of lecithins by high pressure liquid chromatography |
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Authors: | N. A. Porter R. A. Wolf J. R. Nixon |
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Affiliation: | (1) Paul M. Gross Chemical Laboratories, Duke University, 27706 Durham, North Carolina |
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Abstract: | Ten different synthetic lecithins have been analyzed by reverse-phase high pressure liquid chromatography. An empirical lecithin “carbon number” that depends on the total number of carbons and double bonds in the fatty acyl chains in a useful index in predicting retention volumes of lecithins on a nonpolar octadecyl fatty acid column. Commercial egg lecithin is separated into its components by this technique. |
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