RAPD技术对茶树品种鉴别的研究

本研究利用RAPD技术对我国 30多个国家级及省级茶树优良品种进行了分析 ,从 36 0个十聚体随机引物中筛选出了 14 4个有多态性的引物 ,占所用引物的 4 4 %。但同时能在红茶、绿茶两大茶类上产生多态性的引物有5 7个 ,占所筛引物的 16 %左右 ,能在红茶、绿茶、乌龙茶三大类茶树种类上产生多态性的引物只有 38个 ,占所筛引物的 11%左右。 38个能在三大茶类茶树中都能产生多态性引物中仅有 2 2个引物多态性效果好 ,仅占所筛引物的6 %左右。结果发现 :使用OPI14一个引物扩增的DNA指纹图谱能区别开绿茶的 15个品种 ,使用OPI13能区别开绿茶、红茶的 12个品种 (其中绿茶 5个、红茶 7个 ) ,使用OPA10能区别开绿茶、红茶、乌龙茶共计 12个品种。研究表明了利用RAPD技术进行茶树品种鉴别是完全可行的。

Identification of excellent tea germplasms with random amplified polymorphic DNA (RAPD)

More than 30 excellent tea varieties and germplasms were analyzed by u sing RAPD technology. 144primers which amplified polymorphic bands were screene d from 360primers, occupied 44% of all primers. But only 57 primers which amplif i ed polymorphic bands were selected from black tea and green tea, occupied 16% of all primers. and 38 primers which amplified polymorphic bands were selected fro m all of black tea, green tea and wulong tea, occupied 11% of all primers. Among the 38 primers, 22 primers which amplified which amplified polymorphic bands wer e better than the other, occupied 6%. The results illustrated that 15 varieties of green tea were identified using the primer OPI14, 12 varieties of green tea a nd black tea were identified using the primer OPI13, and 12 varieties of black t ea, green tea and wulong tea were identified using the primer OPA10. The study d emonstrated that it was practical and effective to identify tea germplasms with RAPD.