Solid respirometry to characterize nitrification kinetics: a better insight for modelling nitrogen conversion in vertical flow constructed wetlands |
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Authors: | Morvannou Ania Choubert Jean-Marc Vanclooster Marnik Molle Pascal |
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Affiliation: | a Cemagref, UR MALY, 3 bis quai Chauveau - CP 220, F 69336 Lyon, France b Earth and Life Institute Environmental Sciences, Université Catholique de Louvain, Croix du Sud 2 Box 2, B 1348 Louvain-la-Neuve, Belgium |
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Abstract: | We developed an original method to measure nitrification rates at different depths of a vertical flow constructed wetland (VFCW) with variable contents of organic matter (sludge, colonized gravel). The method was adapted for organic matter sampled in constructed wetland (sludge, colonized gravel) operated under partially saturated conditions and is based on respirometric principles. Measurements were performed on a reactor, containing a mixture of organic matter (sludge, colonized gravel) mixed with a bulking agent (wood), on which an ammonium-containing liquid was applied. The oxygen demand was determined from analysing oxygen concentration of the gas passing through the reactor with an on-line analyzer equipped with a paramagnetic detector. Within this paper we present the overall methodology, the factors influencing the measurement (sample volume, nature and concentration of the applied liquid, number of successive applications), and the robustness of the method. The combination of this new method with a mass balance approach also allowed determining the concentration and maximum growth rate of the autotrophic biomass in different layers of a VFCW. These latter parameters are essential inputs for the VFCW plant modelling. |
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Keywords: | Nitrification Solid respirometry Vertical flow constructed wetlands |
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