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产海藻糖合酶菌株发酵培养基的研究
引用本文:薛璐,马莺.产海藻糖合酶菌株发酵培养基的研究[J].食品与发酵工业,2002,28(7):34-36.
作者姓名:薛璐  马莺
作者单位:1. 哈尔滨工业大学生命科学与工程系,哈尔滨,150001
2. 东北农业大学食品学院,哈尔滨,150030
摘    要:在已筛选出产海藻糖合酶菌株———恶臭假单胞杆菌的基础上 ,研究了该菌株最佳发酵培养基。恶臭假单胞杆菌H76的最适碳源为麦芽糖和葡萄糖 ,最适氮源为蛋白胨和酵母粉 ,无机盐为MgSO4 ·7H2 O ;该菌株产酶最佳培养基配方为 :麦芽糖 3 % ,葡萄糖 3 % ,Mg SO4 7H2 O 0 2 % ,蛋白胨 2 % ,酵母粉 0 7%。

关 键 词:恶臭假单胞杆菌  海藻糖合酶  发酵培养基
修稿时间:2002年3月22日

Studies on the Fermentation Medium of the Trehalose Synthase-Producing Strain
Xue Lu,Ma Ying.Studies on the Fermentation Medium of the Trehalose Synthase-Producing Strain[J].Food and Fermentation Industries,2002,28(7):34-36.
Authors:Xue Lu  Ma Ying
Affiliation:Xue Lu 1 Ma Ying 2 1
Abstract:Trehalose is a natural functional disaccharide It could be widely used in foodstuff,pharmaceuticals,cosmetic and agriculture Trehalose synthase can convert maltose into trehalose by the intermolecular transglucosylation And it could be used in the scale production of trehalose This study mainly discussed the fermentation medium of Pseudomonas putida H76,which can produce trehalose synthase The results showed that the best carbon source for Pseudomonas putida H76 is maltose and glucose,the best source for the strain is peptone and yeast extraction,the best inorganic source is MgSO 4·7H 2O The formula of culture medium was optimized as:maltose 3%,glucose 3%,peptone 2%,yeast extraction 0 7%,MgSO 4·7H 2O 0 2%
Keywords:Pseudomonas  putida  trehalose  synthase  fermentation  medium
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