| 东北红豆杉枝叶中紫杉醇和10-脱乙酰巴卡亭的分离纯化及检测 |
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| 引用本文： | 王小刚,惠俊峰,雷闫盈. 东北红豆杉枝叶中紫杉醇和10-脱乙酰巴卡亭的分离纯化及检测[J]. 食品与药品, 2006, 8(12): 35-37 |
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| 作者姓名： | 王小刚 惠俊峰 雷闫盈 |
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| 作者单位： | 西北大学化工学院,陕西,西安,710069 |
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| 摘 要： | 目的用HPLC法对东北红豆杉枝叶中的紫杉醇和10-脱乙酰巴卡亭(10-DAB)进行分离、纯化并对其进行检测。方法采用提取的办法从红豆杉枝叶中粗提紫杉醇和10-DAB;用硅胶色谱柱对粗提物进行分离纯化并进行重结晶;用HPLC法对纯化物进行检测。结果建立了HPLC法,对紫杉醇和10-DAB进行分离、纯化及检测。结论方法简便、准确度高、重现性好。
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| 关 键 词： | 红豆杉 紫杉醇 10-脱乙酰巴卡亭 |
| 文章编号： | 1672-979X(2006)12-0035-03 |
| 修稿时间： | 2006-08-03 |
Separation, Purification and Determination of Paclitaxel and 10-Deacetyl Baccatin from Taxus cuspidata Leaves |
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| WANG Xiao-gang,XI Jun-feng,LEI Yan-ying. Separation, Purification and Determination of Paclitaxel and 10-Deacetyl Baccatin from Taxus cuspidata Leaves[J]. Food and Drug, 2006, 8(12): 35-37 |
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| Authors: | WANG Xiao-gang XI Jun-feng LEI Yan-ying |
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| Abstract: | Objective Paclitaxel and 10-deacetyl baccatin from Taxus cuspidata leaves were separated,purified and determined by HPLC.Methods Paclitaxel and 10-deacetyl baccatin were extracted crudely from Taxus cuspidata leaves,then the crude extracts were separated and purified by a silica gel chromatographic column.Purified compounds were recrystallized.Finally,the compounds were determined by HPLC.Results HPLC method was established for the separation,purification and determination of paclitaxel and 10-deacetyl baccatin.Conclusion The method is convenient,accurate and reproductive. |
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| Keywords: | HPLC |
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