Structural characterisation of polysaccharides from Tricholoma matsutake and their antioxidant and antitumour activities |
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| Authors: | Lijun You Qing Gao Mengying Feng Bao Yang Jiaoyan Ren Longjian Gu Chun Cui Mouming Zhao |
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| Affiliation: | 1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China;2. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China |
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| Abstract: | In this study, polysaccharides from Tricholoma matsutake (TM-P) were purified using a DEAE-Sepharose fast flow column and three polysaccharide fractions (TM-P1, TM-P2 and TM-P3) were obtained. The chemical composition and structural characteristics of TM-P2 were quite different from those of TM-P1 and TM-P3. TM-P2 consisted of glucose, galactose and mannose with a molar ratio of 5.9:1.1:1.0. The glycosidic linkages were mainly composed of 1,6- and 1-linked glucose. Furthermore, TM-P2 showed the strongest in vitro antioxidant and antitumour activities. The oxygen radical absorbance capacity (ORAC) of TM-P2 was 2100.44 μmol Trolox/g. The antiproliferative activities of TM-P2 (4.0 mg/ml) on the growth of HepG2 and A549 cells were 67.98% and 59.04%, respectively. |
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| Keywords: | Tricholoma matsutake Polysaccharides Antioxidant activity Antitumour activity Antiproliferative activity |
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