Phenylalanine ammonia lyase (PAL) enzyme activity and antioxidant properties of some cyanobacteria isolates |
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Authors: | Selcen Babaoğlu Aydaş Sahlan Ozturk Belma Aslım |
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Affiliation: | 1. Gazi University, Vocational High School of Health Services, 06830 Ankara/Gölba??, Turkey;2. Nev?ehir University Faculty of Science and Art Faculty, Department of Biology, 50300 Nev?ehir, Turkey;3. Gazi University, Molecular Biology Research Center, 06830 Ankara/Gölba??, Turkey |
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Abstract: | In the present study, six cyanobacteria isolates were evaluated for the PAL enzyme activity, and their methanol extracts were assessed for the total phenolic amount and other antioxidant parameters. Synechocystis sp. BASO444 and Synechocystis sp. BASO673 isolates with high levels of total phenols (66.0 ± 1.2 μg/mg, 78.1 ± 1.8 μg/mg, respectively) also showed high levels of PAL activities (20.5 ± 3.1 U/mg protein, 17.2 ± 2.3 U/mg protein, respectively) and strong antioxidant activities. To understand the effect of l-phenylalanine (l-phe) on the PAL activity, total phenolic amount, and phenolic constituents, isolates were evaluated with 100 mg/l l-phe. While PAL activities exhibited no significant change with l-phe addition, total phenolic amount of the isolates significantly increased. HPLC analysis revealed gallic acid, trans-cinnamic acid, p-coumaric acid, and ferulic acid as the main compounds. Results suggested that the two isolate mights be an important source for the l-phe inducible phenolic compounds. |
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Keywords: | Cyanobacteria Phenylalanine ammonia lyase (PAL) Total phenolics Antioxidant activity |
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