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Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor
Authors:Riccarda Antiochia  Giuliana Vinci  Lo Gorton
Affiliation:1. Department of Chemistry and Drug Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy;2. Department of Management, Sapienza University of Rome, Via C. Laurenziano 9, 00161 Rome, Italy;3. Department of Analytical Chemistry/Biochemistry and Structural Biology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
Abstract:This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of 1 μM, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 μA cm−2 mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s).
Keywords:Fructose   Food analysis   Biosensor   Fructose dehydrogenase   Osmium redox polymer
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