Monitoring the effect of high pressure and transglutaminase treatment of milk on the evolution of flavour compounds during lactic acid fermentation using PTR-ToF-MS |
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Authors: | Maria Tsevdou Christos Soukoulis Luca Cappellin Flavia Gasperi Petros S. Taoukis Franco Biasioli |
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Affiliation: | 1. Laboratory of Food Chemistry and Technology, School of Chemical Engineering, National Technical University of Athens, Polytechnioupoli Zografou, Zografou 15780, Athens, Greece;2. IASMA Research and Innovation Centre, Foundation Edmund Mach, Food Quality and Nutrition Area, via Mach 1, 38100 San Michele all’ Adige, Trento, Italy |
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Abstract: | In this study, the effects of thermal or high hydrostatic pressure (HHP) treatment of a milk base in the absence or presence of a transglutaminase (TGase) protein cross-linking step on the flavour development of yoghurt were investigated. The presence of several tentatively identified volatile flavour compounds (VOCs), both during the enzymatic treatment and the lactic acid fermentation of the milk base, were monitored using a proton transfer reaction time-of-flight mass spectrometer (PTR-ToF-MS). The formation of the major flavour compounds (acetaldehyde, diacetyl, acetoin, and 2-butanone) followed a sigmoidal trend described by the modified Gompertz model. The HHP treatment of milk increased significantly the volatile compound formation rate whereas it did not affect the duration of the lag phase of formation, with the exception of acetaldehyde and diacetyl formation. On the contrary, the TGase cross-linking of milk did not significantly modify the formation rate of the volatile compounds but shortened the duration of the lag phase of their formation. |
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Keywords: | PTR-ToF-MS VOCs Protein cross-linking High pressure Kinetic models |
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