合欢皮提取物对黑曲霉抑菌活性的研究

采用滤纸片法和生长速率法,测定了合欢皮乙醇提取物对4种常见菌种的抑菌活性;结果表明:合欢皮乙醇提取物对金黄色葡萄球菌的最低抑制浓度(MIC)为20mg/mL,对黑曲霉的抑制中浓度(EC50)为12.43mg/mL,即对黑曲霉抑菌效果较好。再进一步采用生长速率法,测定了合欢皮4种萃取物对黑曲霉(Aspergillus niger)的抑菌活性。4种萃取物中,正丁醇相对黑曲霉抑菌效果最好,其EC50为7.68mg/mL。结论:合欢皮乙醇提取物对黑曲霉的有效抑菌成分主要集中于正丁醇相,对于开发天然食品防腐剂有很大价值与潜力。 

Study on the antimicrobial activity of Albizia julibrissin Durazz. bark extract against Aspergillus niger

The antimicrobial activities of the ethanolic extract of A. julibrissin Durazz. bark against four common microorganisms were evaluated using paper disc method and mycelial growth inhibition method. The results showed that the ethanolic extract had a minimal inhibitory concentration (MIC) of 20mg/mL against Staphylococcus aureus and a median effective concentration (EC50) of 12.43mg/mL against Aspergillus niger. These results indicated that the antimicrobial activity of the ethanolic extract against A. niger was higher than that against three other microorganisms. Then the four phases of the extract were tested against A. niger using mycelial growth inhibition method. The n-butanol phase was the most effective against A. niger among the four phases and its EC50 was 7.68mg/mL. In conclusion, the bioactive constituents were mainly in the n-butanol phase and these results provided people with great value and potential in tapping natural food preservatives.