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The effect of ensiling on PCR-based detection of genetically modified Bt maize |
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| Authors: | Christine Hupfer Johann Mayer Helmut Hotzel Konrad Sachse K.-H. Engel |
| Affiliation: | Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universit?t München, Am Forum 2, D-85350 Freising-Weihenstephan, Germany, DE Bayerische Landesanstalt für Tierzucht, Prof.-Dürrw?chter-Platz 1, D-85586 Poing-Grub, Germany, DE Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterin?rmedizin, Naumburger Strasse 96a, D-07743 Jena, Germany, DE |
| Abstract: | The detection of the genetic modification in silage obtained from insect-resistant Bt maize by means of the polymerase chain reaction (PCR) is described. The detectability of the transgene was shown to be dependent on the length of the genomic target sequence chosen for amplication by the PCR. By amplifying a Bt-maize-specific DNA sequence of 211 bp the genetic modification was detected up to 7 months after ensilage. The effect of maize DNA degradation in the course of the ensilage on the detectability of target sequences was demonstrated in model experiments. Received: 17 December 1998 |
| Keywords: | Polymerase chain reaction Genetically modified maize Detection Silage |
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